hbegf  (Bioss)

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Heparan sulfate as an attachment factor for ASFV is restricted by host HB-EGF

doi: 10.1007/s00018-026-06188-z

Figure Lengend Snippet: Host HB-EGF inhibits ASFV infection by blocking viral attachment and internalization in vitro. A PAMs were infected with purified ASFV-WT (MOI = 2) at 37 °C for 0, 6, 12, and 24 h, respectively. The HB-EGF mRNA expression levels were detected by qPCR. MA-104 cells were transfected with HB-EGF for 24 h, followed by infection with ASFV-WT (MOI = 0.1) at 37 °C for 24 h. The ASFV infection was detected by western blot ( B ) and qPCR ( C ). HB-EGF stable expression MA-104 cells were infected with ASFV-WT (MOI = 0.1) at 37 °C for 24 h. The ASFV infection was detected by western blot ( D ) and qPCR ( E ). HB-EGF-KD MA-104 cells were infected with ASFV-WT (MOI = 0.1) at 37 °C for 24 h. The ASFV infection was detected by western blot ( F ) and qPCR ( G ). The attachment ( H ) and internalization ( I ) of ASFV in Wild-type, HB-EGF stable expression, and HB-EGF-KD MA-104 cells were detected by qPCR. The attachment ( J ) and internalization ( K ) of DiD-ASFV were observed by ZEISS LSM980. The cell membrane stained with green markers was used as a reference, where the cell membrane was colocalized (red) with the binding virus. The attachment ( L ) and internalization ( M ) were counted. More than 100 cells were counted per sample, and the number of cells that bound DiD-ASFV was calculated

Article Snippet: Rabbit anti-HB-EGF antibody was purchased from Abclonal (Wuhan, China).

Techniques: Infection, Blocking Assay, In Vitro, Purification, Expressing, Transfection, Western Blot, Membrane, Staining, Binding Assay, Virus

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Heparan sulfate as an attachment factor for ASFV is restricted by host HB-EGF

doi: 10.1007/s00018-026-06188-z

Figure Lengend Snippet: HB-EGF inhibits ASFV infection by the Heparin Binding domain. Schematic diagram of HB-EGF domain structure ( A ) and the truncation mutants ( B ) used to determine the antiviral domain. MA-104 cells were transfected with HB-EGF-Full, HB-EGF-ΔED, and HB-EGF-ΔHBD, followed by infection with ASFV-WT (MOI = 0.1) at 37 °C for 24 h. The ASFV infection was detected by western blot ( C ) and qPCR ( D ). Schematic diagram of HBD-derived peptide sequence ( E ). The affinity of the peptide for heparin ( F ) and heparan sulfate ( G ) was detected by Bio-Layer Interferometry. PAMs were pretreated with different concentrations of peptide, followed by infection with ASFV-WT (MOI = 0.1) at 37 °C for 24 h ( H ). ASFV p72 protein and mRNA expression were assessed by western blot ( I ) and qPCR ( J ). The progeny virus production was quantified by the HDA50 assay ( K ). Recombinant HB-EGF (200 ng/ml) was used to pretreat PAMs at 37 °C for 6 h in the presence or absence of Heparin (200 ng/ml), followed by infection with ASFV-WT/GFP (MOI = 0.1) at 37 °C for the indicated time points ( L ). ASFV infection was quantified by western blot ( M ), fluorescence microscopy ( N , O ), and HAD50 assay ( P )

Article Snippet: Rabbit anti-HB-EGF antibody was purchased from Abclonal (Wuhan, China).

Techniques: Infection, Binding Assay, Transfection, Western Blot, Derivative Assay, Sequencing, Expressing, Virus, Recombinant, Fluorescence, Microscopy

Journal: PLOS One

Article Title: Role of endothelial cell markers in prognosis of hepatocellular carcinoma: Integrating bioinformatics analysis and experimental validation

doi: 10.1371/journal.pone.0331580

Figure Lengend Snippet: (A-E) Representative immunohistochemical images showing the expression of FDPS, FKBP1A, HB-EGF, KLRB1 ( CD161 ), and NDRG1 in HCC tissue specimens. (F–J) Corresponding images of target protein expression in normal liver tissue. Positive staining was consistently observed in HCC samples, with notable localization to sinusoidal endothelial cells, whereas expression in normal liver tissue was largely negative or weakly positive.

Article Snippet: The following monoclonal antibodies, A16365 and A4050, which specifically target HB-EGF and NDRG1, respectively, were acquired from ABclonal (Wuhan, Hubei, China).

Techniques: Immunohistochemical staining, Expressing, Staining